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Fisher Scientific high capacity cdna reverse transcription kit
High Capacity Cdna Reverse Transcription Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+capacity+cdna+reverse+transcription+kit/capacity+cdna+high+kit+reverse+transcription/pm42100825-61-56-81
Average 86 stars, based on 1 article reviews
high capacity cdna reverse transcription kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Reverse Transcription:

Article Title: An epigenomic investigation of atrial fibrillation in a matched left and right atrial human cohort
Article Snippet: RNA integrity was assessed with the RNA 6000 Nano Kit for Bioanalyzer 2100 (Agilent). .. 1000 ng of RNA were reversed transcribed into complementary DNA (cDNA) using the High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific), and RT-qPCR reactions were performed using 5 ng of template cDNA. ..

Article Title: Impacts of ketogenic diet intervention on cardiometabolic outcomes in obese, dysglycemic mice.
Article Snippet: Effluent insulin concentration was measured with Mouse Ultrasensitive or High Range Insulin ELISA (ALPCO, 80-INSMSU-E01 or 80-INSMSH). .. Liver RNA was isolated and cDNA was reverse transcribed with TRIzol Reagent (Ambion) and High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific 43-688-14), respectively, per manufacturers protocols. mRNA quantity was determined with TaqMan Fast Advanced Master Mix (Applied Biosystems 4444557) using standard curve method (Quantstudio 5) and was normalized to Ppia or Tbp. .. Aorta RNA was isolated with TRIzol Reagent (Ambion) and prepared for Nanostring gene expression analysis using manufacturers protocol.

Article Title: Impacts of ketogenic diet intervention on cardiometabolic outcomes in obese, dysglycemic mice
Article Snippet: Effluent insulin concentration was measured with Mouse Ultrasensitive or High Range Insulin ELISA (ALPCO, 80-INSMSU-E01 or 80-INSMSH). .. Liver RNA was isolated and cDNA was reverse transcribed with TRIzol Reagent (Ambion) and High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific 43-688-14), respectively, per manufacturers protocols. mRNA quantity was determined with TaqMan Fast Advanced Master Mix (Applied Biosystems 4444557) using standard curve method (Quantstudio 5) and was normalized to Ppia or Tbp . .. Aorta RNA was isolated with TRIzol Reagent (Ambion) and prepared for Nanostring gene expression analysis using manufacturers protocol.

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: OKN4395, a first-in-class EP2/EP4/DP1 triple antagonist reprograms prostanoid-driven immunosuppression to restore antitumor immunity
Article Snippet: RNA was extracted from CD4 + T cells, CD8 + T cells or NK cells that had been isolated from healthy donor PBMCs or from HEK293T cells (CRL-3216, ATCC) using the RNeasy Mini kit (74106, Qiagen). .. RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (4374967, Fisher Scientific). qPCR was performed in a QuantStudio 7 Pro using the TaqMan Fast Advanced Master Mix (4444557, Fisher Scientific) and the following TaqMan assay ID (Thermo Fisher Scientific) specific for the respective human genes of interest: PTGER1 (Hs00168752_m1), PTGER2 (Hs00168754_m1), PTGER3 (Hs00168755_m1), PTGER4 (Hs00168761_m1), PTGDR (Hs00235003_m1), PTGDR2 (Hs00173717_m1). ..

Article Title: Mitochondrial Transplantation as a New Therapeutic Approach Against Cardiac and Renal Consequences in Male Rats With Myocardial Infarction.
Article Snippet: .. Total RNA was isolated using Trizol Reagent (Fisher Scientific Inc., Waltham, MA, USA) and was reverse- transcribed into cDNA using the High- Capacity cDNA Reverse Transcription Kit (Fisher Scientific Inc., Waltham, MA, USA). ..

Article Title: An epigenomic investigation of atrial fibrillation in a matched left and right atrial human cohort.
Article Snippet: RNA integrity was assessed with the RNA 6000 Nano Kit for Bioanalyzer 2100 (Agilent). .. 1000 ng of RNA were reversed transcribed into complementary DNA (cDNA) using the High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific), and RT-qPCR reactions were performed using 5 ng of template cDNA. ..

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.

Quantitative RT-PCR:

Article Title: An epigenomic investigation of atrial fibrillation in a matched left and right atrial human cohort
Article Snippet: RNA integrity was assessed with the RNA 6000 Nano Kit for Bioanalyzer 2100 (Agilent). .. 1000 ng of RNA were reversed transcribed into complementary DNA (cDNA) using the High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific), and RT-qPCR reactions were performed using 5 ng of template cDNA. ..

Article Title: An epigenomic investigation of atrial fibrillation in a matched left and right atrial human cohort.
Article Snippet: RNA integrity was assessed with the RNA 6000 Nano Kit for Bioanalyzer 2100 (Agilent). .. 1000 ng of RNA were reversed transcribed into complementary DNA (cDNA) using the High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific), and RT-qPCR reactions were performed using 5 ng of template cDNA. ..

Isolation:

Article Title: Impacts of ketogenic diet intervention on cardiometabolic outcomes in obese, dysglycemic mice.
Article Snippet: Effluent insulin concentration was measured with Mouse Ultrasensitive or High Range Insulin ELISA (ALPCO, 80-INSMSU-E01 or 80-INSMSH). .. Liver RNA was isolated and cDNA was reverse transcribed with TRIzol Reagent (Ambion) and High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific 43-688-14), respectively, per manufacturers protocols. mRNA quantity was determined with TaqMan Fast Advanced Master Mix (Applied Biosystems 4444557) using standard curve method (Quantstudio 5) and was normalized to Ppia or Tbp. .. Aorta RNA was isolated with TRIzol Reagent (Ambion) and prepared for Nanostring gene expression analysis using manufacturers protocol.

Article Title: Impacts of ketogenic diet intervention on cardiometabolic outcomes in obese, dysglycemic mice
Article Snippet: Effluent insulin concentration was measured with Mouse Ultrasensitive or High Range Insulin ELISA (ALPCO, 80-INSMSU-E01 or 80-INSMSH). .. Liver RNA was isolated and cDNA was reverse transcribed with TRIzol Reagent (Ambion) and High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific 43-688-14), respectively, per manufacturers protocols. mRNA quantity was determined with TaqMan Fast Advanced Master Mix (Applied Biosystems 4444557) using standard curve method (Quantstudio 5) and was normalized to Ppia or Tbp . .. Aorta RNA was isolated with TRIzol Reagent (Ambion) and prepared for Nanostring gene expression analysis using manufacturers protocol.

Article Title: Mitochondrial Transplantation as a New Therapeutic Approach Against Cardiac and Renal Consequences in Male Rats With Myocardial Infarction.
Article Snippet: .. Total RNA was isolated using Trizol Reagent (Fisher Scientific Inc., Waltham, MA, USA) and was reverse- transcribed into cDNA using the High- Capacity cDNA Reverse Transcription Kit (Fisher Scientific Inc., Waltham, MA, USA). ..

Viscosity:

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.

Modification:

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.

dsDNA Assay:

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation
Article Snippet: Sodium acetate and glacial acetic acid were purchased from Scharlab (Spain). .. Low viscosity alginic acid sodium salt (Sodium alginate) (SA), Hank’s balanced salt solution (HBSS, no calcium, no magnesium, no phenol red), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin solution (P/S), horseradish peroxidase (HRP), TRIzol® Reagent, AlamarBlueTMcell viability reagent, Quant-iTTM PicoGreenTM dsDNA assay kit, NucBlueTM Live ReadyProbesTM Reagent (Hoechst 33,342), Live/DeadTM Viability/Cytotoxicity Kit, High Capacity cDNA Reverse Transcription Kit, Taqman Fast Advanced Master Mix, Rhodamine Phalloidin, Paraformaldehyde and Donkey anti-Rabbit secondary antibody Alexa Fluor 488 were procured from Fisher Scientific (Spain). .. Sodium hyaluronates (Hyaluronic acid) (HA, MW 100 kDa and 1 MDa) were purchase from Lifecore ® Biomedical (USA).

Article Title: Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.
Article Snippet: Aim: To investigate the role of hydrogel composition and nanozymes incorporation on the inflammatory response of encapsulated murine microglia.. Methods: Sodium alginate (SA) hydrogels containing i) hyaluronic acid (HA) of low-100 kDa or high-1 MDa molecular weight and ii) Mn3O4 nanoparticles (Mn3O4-NPs) were prepared and characterized.. The inflammatory response of encapsulated microglia was assessed.

Real-time Polymerase Chain Reaction:

Article Title: OKN4395, a first-in-class EP2/EP4/DP1 triple antagonist reprograms prostanoid-driven immunosuppression to restore antitumor immunity
Article Snippet: RNA was extracted from CD4 + T cells, CD8 + T cells or NK cells that had been isolated from healthy donor PBMCs or from HEK293T cells (CRL-3216, ATCC) using the RNeasy Mini kit (74106, Qiagen). .. RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (4374967, Fisher Scientific). qPCR was performed in a QuantStudio 7 Pro using the TaqMan Fast Advanced Master Mix (4444557, Fisher Scientific) and the following TaqMan assay ID (Thermo Fisher Scientific) specific for the respective human genes of interest: PTGER1 (Hs00168752_m1), PTGER2 (Hs00168754_m1), PTGER3 (Hs00168755_m1), PTGER4 (Hs00168761_m1), PTGDR (Hs00235003_m1), PTGDR2 (Hs00173717_m1). ..

TaqMan Assay:

Article Title: OKN4395, a first-in-class EP2/EP4/DP1 triple antagonist reprograms prostanoid-driven immunosuppression to restore antitumor immunity
Article Snippet: RNA was extracted from CD4 + T cells, CD8 + T cells or NK cells that had been isolated from healthy donor PBMCs or from HEK293T cells (CRL-3216, ATCC) using the RNeasy Mini kit (74106, Qiagen). .. RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (4374967, Fisher Scientific). qPCR was performed in a QuantStudio 7 Pro using the TaqMan Fast Advanced Master Mix (4444557, Fisher Scientific) and the following TaqMan assay ID (Thermo Fisher Scientific) specific for the respective human genes of interest: PTGER1 (Hs00168752_m1), PTGER2 (Hs00168754_m1), PTGER3 (Hs00168755_m1), PTGER4 (Hs00168761_m1), PTGDR (Hs00235003_m1), PTGDR2 (Hs00173717_m1). ..



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